Bacteria
- Plasmid construction
- Recombinant protein purification
Yeast
- Protein-protein interaction assays:
- 1. Ras Recruitment System
- 2. Two Hybrid System
Tissue Culture
- Transfection
- Retrovirus Infection
- Gene expression assay
- ChIP
- Protein extraction
- Functinal assay
Mice
- Transgenic Mice
- KO Mice
- Mini Osmotic Pumps implantation
- Caridiomyocytes isolation
- Liver Cancer
- Xenograft Tumor Models
- Bone Marrow Transplantation
- EKG and Echoradiography
Methods:
- Molecular Biology Techniques
- Western blot analysis
- Protein-protein interactions
- Electromobility shift assay
- Imaging
- Cell stainings
- Real time PCR
- Reporter Gene Assay
- Tissue Section